Ultra Pure Grade
Purity (enzymatic): >99.0%
Purity (UV): >99.0%
Inhibitors (enzymatic): <0.1%
Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. For best results, protect from light and allow to reach ambient temperature before use.