Jacalin lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains it's carbohydrate-binding specificity of T-antigen disaccharide Galβ1,3GalNAc. Potent inhibitors of jacalin include D-galactose, β-Met-Gal and 2-deoxy-α-D-galactose. Cy5, when bound to Artocarpus integrifolia (Jacalin) Lectin (AIA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.
- Source: Artocarpus integrifolia (Jacalin)
- Protein Concentration (Based on OD 280): 1 mg purified AIA Cy5 / 1 mL buffer
- Carbohydrate Specificity: Galβ3GalNAc
- Inhibitory Carbohydrate: Galactose or Melibiose
- Mitogenic Activity: Yes
- Divalent Ions Required: None
- Conjugation: Cy5
- Excitation: Maximum at 650 nm
- Emission: Maximum at 670 nm
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.