Arachis hypogaea Lectin (PNA) - FITC (Fluorescein)

Arachis hypogaea Lectin (PNA) - FITC (Fluorescein)
Arachis hypogaea (Peanut) Lectin (PNA) - FITC (Fluorescein)
Product image 1Arachis hypogaea Lectin (PNA) - FITC (Fluorescein)
Product image 2Arachis hypogaea (Peanut) Lectin (PNA) - FITC (Fluorescein)

Price: Regular price $98.00

SKU#: 21761000-1

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA.

Although extracts of Arachis hypogaea do not agglutinate untreated or trypsin-treated human erythrocytes, PNA has been known to agglutinate neuraminidase-treated human red blood cells. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Arachis hypogaea (Peanut) Lectin (PNA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. 

Technical Specifications

Abbreviation: PNA
Material Source:: Peanut
Conjugate:: FITC (Fluorescein)
Concentration:: 5 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Galactose
Fluorescence:: Green
Divalent Ions:: Ca++, Mg++
Excitation:: 495 nm
Emission:: 515 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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