Arachis hypogaea Lectin (PNA) - Biotinylated
Price: Regular price $189.00
Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Arachis hypogaea (Peanut) Lectin (PNA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
- Source: (Peanut)
- Activity: Less than 1 μg/ml will agglutinate human erythrocytes after neuraminidase treatment of the cells.
- Protein Concentration (Based on OD 280): 5 mg purified PNA - Biotin / 1 mL buffer
- Carbohydrate Specificity: Galactose
- Inhibitory Carbohydrate: Lactose
- Divalent Ions Required: Ca++, Mg++
- Conjugation: Biotin
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.
|Conjugate:||:||Biotinyl N- Hydroxysuccinimide Ester (Biotin)|
|Molecular Weight:||:||16 kDa|
|Purity:||:||High Purity Grade|
|Shelf Life:||:||1 year|
|Blood Group Specificity:||:||Non-specific|
|Preferred Sugar Specificity:||:||Galβ3GalNAc|
|Inhibiting or Eluting Sugar:||:||Galactose|
|Divalent Ions:||:||Ca++, Mg++|
|Lyophilized or Liquid||:||Liquid|
Technical DocumentationSafety Data Sheet (SDS)
Certificates of Analysis (COA)