Arachis hypogaea Lectin (PNA) - Biotinylated

Arachis hypogaea Lectin (PNA) - Biotinylated

Price: Regular price $267.67

SKU#: 21510020-1

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Arachis hypogaea (Peanut) Lectin (PNA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Specifications:

  • Source: (Peanut)
  • Activity: Less than 1 μg/ml will agglutinate human erythrocytes after neuraminidase treatment of the cells.
  • Protein Concentration (Based on OD 280): 5 mg purified PNA - Biotin / 1 mL buffer
  • Carbohydrate Specificity: Galactose
  • Inhibitory Carbohydrate: Lactose
  • Divalent Ions Required: Ca++, Mg++
  • Conjugation: Biotin

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.

Technical Specifications

Abbreviation: PNA
Material Source:: Peanut
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 5 mg/mL
Molecular Weight:: 16 kDa
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Galactose
Divalent Ions:: Ca++, Mg++
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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