Amaranthus caudatus Lectin (ACL/ACA) - Biotinylated
Price: Regular price $0.00
Product Description
Affinity-purified Amaranthus caudatus (ACL/ACA) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the “T” antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.
Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated ACL has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
Applications: Glycobiology, Immunohistochemistry, Western blotting, ELISA
Recommended Usage: Recommended dilution is 0.5 – 10 µg/ml in 1XPBS. Less than 05µg/ml of ACL can agglutinate neuraminidase treated human erythrocyte.
Technical Specifications
| Abbreviation | : | ACL/ACA |
| Material Source: | : | Tassle flower |
| Conjugate: | : | Biotinyl N- Hydroxysuccinimide Ester (Biotin) |
| Concentration: | : | 1 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Disialyzed Fetuin |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
