Affinity-purified Amaranthus caudatus (ACL) (SKU: 21510158) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oliogsacharrides containing the galactosyl disacharride Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 position of galactose in the "T" antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.
Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated ACL has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
• Source: Amaranthus caudatus (Tassle flower)
• Activity: ACL agglutinates human ABO and sheep, goat, rat, rabbit and guinea-pig erythrocytes.
• Carbohydrate Specificity: Galß1-3GalNAc
• Inhibitory Carbohydrate: Disialyzed Fetuin
• Divalent ions required: None
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use. Clarify by centrifugation, if needed.