Allium sativum Lectin (ASA) - DyLight 488
Price: Regular price $396.25
Product Description
Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research
Dylight 488 can be used to visualize the binding pattern of Allium sativum Lectin (ASA) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that works over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.
Recommended Usage: 1XPBS can be used for reconstitution/dilution. Recommended dilution is 0.5 - 10 µg /ml in 1XPBS.
Technical Specifications
| Abbreviation | : | ASA |
| Material Source: | : | Garlic Bulb |
| Conjugate: | : | DyLight 488 |
| Concentration: | : | 1 mg/ml |
| Molecular Weight: | : | 25, 48 kDa |
| Appearance Form: | : | Liquid |
| Appearance Color: | : | Green |
| Purity: | : | High Grade Purity |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Rabbit Erythrocytes |
| Preferred Sugar Specificity: | : | D-Mannose |
| Inhibiting or Eluting Sugar: | : | (Man)2(GlcNAc)2 |
| Fluorescence: | : | Green |
| Divalent Ions: | : | None |
| Excitation: | : | 493 |
| Emission: | : | 518 |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Blotting, ELISA, ELLA, Bioconjugation, Cell typing, Glycobiology.
References
1. Ghosh, P., Sen, S., Chakraborty, J., & Das, S. (2016). Monitoring the efficacy of utated Allium sativum leaf lectin in transgenic rice against Rhizoctonia solani. BMC biotechnology, 16, 24. https://doi.org/10.1186/s12896-016-0246-0.
2. Kumar, S., Jitendra, K., Singh, K., Kapoor, V., Sinha, M., Xess, I., Das, S. N., Sharma, S., Singh, T. P., & Dey, S. (2015). Biological Properties and Characterization of ASL50 Protein from Aged Allium sativum Bulbs. Applied biochemistry and biotechnology, 176(7), 1914–1927.