Agaricus bisporus Lectin (ABA/ABL) - Cy5

Agaricus bisporus Lectin (ABA/ABL) - Cy5

Price: Regular price $212.18

SKU#: 21761081-1

Product Description

Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins.

Pure BA was conjugated with Cy5, bound to Agaricus bisporus Lectin (ABA/ABL), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlapping with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Applications: Glycobiology, Immunofluorescence, Immunohistochemistry, Immunocytochemistry

Recommended Usage: Recommended concentrations from 0.5-10 ug/ml in 1XPBS.

Technical Specifications

Abbreviation: ABA/ABL
Material Source:: Mushroom
Conjugate:: Cyanine 5 (Cy5)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galactoseβ1-3N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: Fetuin
Fluorescence:: Red
Divalent Ions:: None Required
Excitation:: 650 nm
Emission:: 670 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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