21511594-1 Lotus tetragonolobus Lectin (LTL) - Alexa Fluor 647

Price: Regular price $240.91

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Product Description

Lotus tetragonolobus Lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid

residues. LTL is a glycoprotein with a molecular weight of 107,000 and consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) Lectin. Reports show that a minimum hemagglutinating dose of 3.3 µg/mL of the purified lectin against neuraminidase-treated human O erythrocytes, 26.4 µg/mL against B erythrocytes, and 105.6 µg/mL against A erythrocytes. Transitional cell carcinoma of the human urinary bladder tend to metastasize when it strongly expresses binding sites for LTL. See other LTL conjugates. This product comes in a lyophilized form and is stored at -20°C. Calcium and manganese ions are required for binding.

Lotus tetragonolobus Lectin conjugated with Alexa Fluor™ 647 is a bright, far red fluorescent dye optimized for stable signal generation in imaging and flow cytometry. The excitation wavelength is ideally suited for the 633 nm laser line, ensuring reliable detection in various applications. This dye is water soluble and maintains pH insensitivity from pH 4 to pH 10, providing dependable performance across a range of experimental conditions. The Alexa Fluor™ 647 conjugate enhances the capabilities for cellular labeling and detection, making it an excellent choice for advanced research applications.

Immunofluorescence, Immunohistology, Immunocytochemistry, Cell typing, ELLISA, Diagnostic tools, Glycobiology.

Technical Specifications

Abbreviation: LTL
Material Source:: L-Fucose
Conjugate:: Alex Fluor 647
Concentration:: 1 mg/ml
Molecular Weight:: 107 kDa
Appearance Form:: Liquid
Appearance Color:: Blue
Purity:: High grade purity
Shelf Life:: 2 years
Blood Group Specificity:: O>A2
Preferred Sugar Specificity:: L-Fucose
Inhibiting or Eluting Sugar:: Methyl x-L-Fucopyranoside
Fluorescence:: Far-red
Excitation:: 650
Emission:: 671
Mitogenic Activity:: None
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

 Immunofluorescence, Immunohistology, Immunocytochemistry, Cell typing, ELLISA, Diagnostic tools, Glycobiology.

References

  1. Allen HJ, Johnson EA. A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus. Carbohydr Res. 1977 Oct;58(2):253-65. doi: 10.1016/s0008-6215(00)84352-9. PMID: 912690.
  2. Yariv J, Kalb AJ, Katchalski E. Isolation of an L-fucose binding protein from Lotus tetragonolobus seed. Nature. 1967 Aug 19;215(5103):890-1.

Technical Documentation

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