21511594-1 Lotus tetragonolobus Lectin (LTL) - Alexa Fluor 647
Price: Regular price $240.91
Product Description
Lotus tetragonolobus Lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid
residues. LTL is a glycoprotein with a molecular weight of 107,000 and consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) Lectin. Reports show that a minimum hemagglutinating dose of 3.3 µg/mL of the purified lectin against neuraminidase-treated human O erythrocytes, 26.4 µg/mL against B erythrocytes, and 105.6 µg/mL against A erythrocytes. Transitional cell carcinoma of the human urinary bladder tend to metastasize when it strongly expresses binding sites for LTL. See other LTL conjugates. This product comes in a lyophilized form and is stored at -20°C. Calcium and manganese ions are required for binding.
Lotus tetragonolobus Lectin conjugated with Alexa Fluor™ 647 is a bright, far red fluorescent dye optimized for stable signal generation in imaging and flow cytometry. The excitation wavelength is ideally suited for the 633 nm laser line, ensuring reliable detection in various applications. This dye is water soluble and maintains pH insensitivity from pH 4 to pH 10, providing dependable performance across a range of experimental conditions. The Alexa Fluor™ 647 conjugate enhances the capabilities for cellular labeling and detection, making it an excellent choice for advanced research applications.
Immunofluorescence, Immunohistology, Immunocytochemistry, Cell typing, ELLISA, Diagnostic tools, Glycobiology.
Technical Specifications
| Abbreviation | : | LTL |
| Material Source: | : | L-Fucose |
| Conjugate: | : | Alex Fluor 647 |
| Concentration: | : | 1 mg/ml |
| Molecular Weight: | : | 107 kDa |
| Appearance Form: | : | Liquid |
| Appearance Color: | : | Blue |
| Purity: | : | High grade purity |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | O>A2 |
| Preferred Sugar Specificity: | : | L-Fucose |
| Inhibiting or Eluting Sugar: | : | Methyl x-L-Fucopyranoside |
| Fluorescence: | : | Far-red |
| Excitation: | : | 650 |
| Emission: | : | 671 |
| Mitogenic Activity: | : | None |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence, Immunohistology, Immunocytochemistry, Cell typing, ELLISA, Diagnostic tools, Glycobiology.
References
- Allen HJ, Johnson EA. A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus. Carbohydr Res. 1977 Oct;58(2):253-65. doi: 10.1016/s0008-6215(00)84352-9. PMID: 912690.
- Yariv J, Kalb AJ, Katchalski E. Isolation of an L-fucose binding protein from Lotus tetragonolobus seed. Nature. 1967 Aug 19;215(5103):890-1.