Solanum tuberosum Lectin (STA/STL) - Pure
Price: Regular price $263.72
Product Description
Solanum tuberosum lectin/agglutinin (STA/STL) is a hydroxyproline-rich glycoprotein isolated from potato and purified by affinity chromatography. This lectin contains 50% carbohydrates, predominantly arabinose; and consists of two subunits. STA/STL binds oligomers of N-acetylglucosamine and some bacterial cell wall oligosaccharides containing N-acetylglucosamine and N-acetylmuramic acid. N-acetyllactosamine is a relatively weak inhibitor of hemagglutination induced by this lectin; though, it does not have a blood group specificity. Solanum tuberosum lectin has been shown to bind two and a half times more to colon cancer cells than normal tissue.
Recommended Usage: Recommended dilutions of 0.5 – 15 μg in 1XPBS. Less than 15 µg/ml will agglutinate human type O erythrocytes. The activity is greater with neuraminidase treated red blood cells.
Technical Specifications
| Abbreviation | : | STA/STL |
| Material Source: | : | Potato |
| Conjugate: | : | None |
| Concentration: | : | 5 mg, 10 mg |
| Molecular Weight: | : | 100 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off - white to brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | GlcNAc |
| Inhibiting or Eluting Sugar: | : | Chitotriose |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized Powder |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Bioconjugations, Blotting, Cell typing, ELISA, Bacterial cell wall binding assay, Glycobiology.
References
- Matsumoto I, Jimbo A, Mizuno Y, Seno N, Jeanloz RW. Purification and characterization of potato lectin. J Biol Chem. 1983 Mar 10;258(5):2886-91.
- Mirka Šafaříková& Ivo Šafařík. One-step partial purification of Solanum tuberosum tuber lectin using magnetic chitosan particles. Biotechnology Letters 2000, 22: 941–945.
- Desai NN, Allen AK. The purification of potato lectin by affinity chromatography on an N,N',N''-triacetylchitotriose-Sepharose matrix. Anal Biochem. 1979 Feb;93(1):88-90.
- Feng Y, Song J, Zhao Z, Zhao F, Yang L, Jiao C. A rapid and effective method for purification of a heat-resistant lectin from potato (Solanum tuberosum) tubers. Glycoconj J. 2018 Aug;35(4):403-409.
