Datura stramonium Lectin (DSA/DSL) - Pure
Price: Regular price $131.59
Product Description
Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two non-identical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (β-1,6) and (β-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urothelial cells.
Recommended Usage: Recommended dilutions of 0.5 – 10 µg/ml in 1XPBS. Human O erythrocytes will agglutinate by 20 – 30 µg/ml of DSA.
Technical Specifications
| Abbreviation | : | DSA/DSL |
| Material Source: | : | Jimson weed |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 5 mg |
| Molecular Weight: | : | 67 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A, B, O |
| Preferred Sugar Specificity: | : | (N-Acetylglucosamine)2-4 |
| Inhibiting or Eluting Sugar: | : | Chitin Hydrolysate |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Western blotting, Mitogenic stimulation, ELISA, ELISPOT, Immunohistochemistry, Immunocytochemistry, Antiproliferative, Glycobiology.
References
- Nishimoto K, Tanaka K, Murakami T, Nakashita H, Sakamoto H, Oguri S. Datura stramonium agglutinin: cloning, molecular characterization and recombinant production in Arabidopsis thaliana. Glycobiology. 2015 Feb;25(2):157-69.
- Hirabayashi J, Lectin Purifications and analysis. Book. 2020. Methods in Molecular Biology(MIMB, volume 2132).
- Kilpatrick DC, Yeoman MM. Purification of the lectin from Datura stramonium. Biochem J. 1978 Dec 1;175(3):1151-3.
Technical Documentation
Product Information SheetSafety Data Sheet (SDS)
Certificates of Analysis (COA)
