Pisum sativum Lectin (PSA/PSL) - Pure
Price: Regular price $210.70
Product Description
Affinity purified pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.
Recommended Usage: Recommended dilutions of 1-20 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. Between 10-100 mg/ml may be required to agglutinate neuraminidase treated red blood cells.
Technical Specifications
| Abbreviation | : | PSA/PSL |
| Material Source: | : | Pea |
| Conjugate: | : | None |
| Concentration: | : | 5 mg, 10 mg |
| Molecular Weight: | : | 50 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | off white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | D-Mannose, D-Glucose |
| Inhibiting or Eluting Sugar: | : | Methyl α-D-Mannopyranoside, Trehalose |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, ELISA, Blotting, Cell typing, Mitogenic stimulation, Bioconjugations, Biomarker study, Purification study of feline T-lymphocytes, Glycobiology.
References
- Kosenko, L. V. Comparative Characteristics of Carbohydrate Binding by Lectins from Broad Bean, Pea, Common Vetch, and Lentil Seeds. Russian Journal of Plant Physiology 49(5):p 641-646, September-October 2002
- El-Araby MM, El-Shatoury EH, Soliman MM, Shaaban HF. Characterization and antimicrobial activity of lectins purified from three Egyptian leguminous seeds. AMB Express. 2020 May 15;10(1):90.
