Sialo-Profiling Kit (Alpha 2,3/2,6)
Price: Regular price $599.85
Product Description
Sialylation plays a significant role in immune responses, cellular communication, pathogen recognition, cell survival, and cardiovascular disease. The differentiation of linkages in sialic acid (alpha 2,3/alpha 2,6) has a crucial role in various diseases, e.g., alpha 2,3 sialic acid is involved in cancer progression, and alpha 2,6 is involved in immune response regulation. The sialylation profiling kit includes four distinct Lectins, enabling detection and analysis of alpha 2,3 and alpha 2,6 sialic acid in cells and tissues. Sambucus nigra Lectin (SNA I) can recognize alpha 2,6 sialic acid on sialylated glycans, whereas MAA II (Maackia amurensis Lectin) can recognize alpha 2,3 on the surface. The other two Lectins (PNA & ECL) can recognize non-sialylated glycans by their unique sugar specificities. This kit provides other reagents and buffers needed for specific analyses, as well as technical support for experimental needs.
Kit Contents:
SKU/ Lectin/ Amount
21510106/ Sambucus nigra Lectin (SNA/EBL I) - Biotinylated/ 0.5 mg
21511113/ Maackia amurensis Lectin (MAA/MAL I+II) - Biotinylated/ 0.5 mg
21510020/ Arachis hypogaea Lectin (PNA) - Biotinylated/ 0.5 mg
21510048/ Erythrina cristagalli Lectin (ECL/ECA) - Biotinylated/ 0.5 mg
40180007/ Streptavidin – FITC conjugate for detection/ 0.5 mg
40120069/ PBS Buffer 1X, pH 7.4/ 50 ml
Specifications
- Sambucus nigra Lectin (SNA/EBL I) - Recognizes alpha 2,6 sialic acids
- Maackia amurensis Lectin (MAA/MAL I+II) - Recognizes alpha 2,3 sialic acids
- Arachis hypogaea Lectin (PNA) - Recognizes T antigen, an O-glycan
- Erythrina Cristagalli Lectin (ECL/ECA) - Recognizes terminal beta 1,4-Gal
Technical Information
- Recommended cell number to use is 2 - 5E6 cells per analysis.
- Recommended Lectin dilution is 5 – 40 µg / mL in PBS Buffer 1X, pH 7.4
- Binding efficiency depends on target cell lines, time of incubations.
- Avoid multiple freeze-thaw cycles.
- Concentrated amounts of Lectin conjugate can cause cytotoxicity.
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze-thaw cycles. It can be stored at 2-8°C for short-term use. Clarify by centrifugation, if needed.
Technical Specifications
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
1.Total/Altered sialylation detection in cellular communication, pathogen recognition, cell survival, and cardiovascular disease (Live cells, Plasma, Serum, Tissues).
2.Lectins, enabling detection and analysis of alpha 2,3 and alpha 2,6 sialic acid in cells and tissues.
3.Detection of T – antigen (O Glycans).
4.Flow/IHC based detection.
References
1.Kaku H, Peumans WJ, Goldstein IJ. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. Arch Biochem Biophys. 1990 Mar;277(2):255-62.
2.Maveyraud L, Niwa H, Guillet V, Svergun DI, Konarev PV, Palmer RA, Peumans WJ, Rougé P, Van Damme EJ, Reynolds CD, Mourey L. Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra. Proteins. 2009 Apr;75(1):89-103.
3.Bum Soo Kim, Kyung Taik Oh,1, Due Hyeon Cho, Yun Jung Kim, Wan Mo Koo, Kwang Hoon Kong, HaHyung Kim. A sialic acid-binding lectin from the legume Maackia fauriei: comparison with lectins from M. amurensis. Plant Science 167 (2004) 1315–1321.
4.Konami Y, Yamamoto K, Osawa T, Irimura T. Strong affinity of Maackia amurensis hemagglutinin (MAH) for sialic acid-containing Ser/Thr-linked carbohydrate chains of N-terminal octapeptides from human glycophorin A. FEBS Lett. 1994 Apr 11;342(3):334-8.
5.Jie Sun, Qing-li Yang*, jie Bi, Chu-shu Zhang, Li-na Yu and Feng Zhu. Purification and Identification of a Natural Lectin from the Seed of Peanut Arachis hypogaea. The Open Materials Science Journal, 2011, 5, 78-82
6.Erber, W. N., Asbahr, H., Meyer, B., Herrmann, R. P., & Davies, J. M. (1992). Peanut agglutinin (lectin from Arachis hypogaea) binding to hemopoietic cells: an immunophenotypic study using a biotin streptavidin technique. Pathology, 24(3), 173–176.
7.Datta TK, Basu PS. Identification, isolation and some properties of lectin from the seeds of Indian coral tree [Erythrina variegata (Linn.) var. orientalis (Linn.) Merrill]. Biochem J. 1981 Sep 1;197(3):751-3.
8.Iglesias JL, Lis H, Sharon N. Purification and properties of a D-galactose/N-acetyl-D-galactosamine-specific lectin from Erythrina cristagalli. Eur J Biochem. 1982 Apr 1;123(2):247-52.
9.Harmer IJ, Samuel D. The FITC-anti-FITC system is a sensitive alternative to biotin-streptavidin in ELISA. J Immunol Methods. 1989 Aug 15;122(1):115-2.
