Lotus tetragonolobus Lectin (LTL) - Pure
Price: Regular price $187.27
Product Description
Lotus tetragonolobus Lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid
residues. LTL is a glycoprotein with a molecular weight of 107,000 and consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) Lectin. Reports show that a minimum hemagglutinating dose of 3.3 µg/mL of the purified lectin against neuraminidase-treated human O erythrocytes, 26.4 µg/mL against B erythrocytes, and 105.6 µg/mL against A erythrocytes. Transitional cell carcinoma of the human urinary bladder tend to metastasize when it strongly expresses binding sites for LTL. See other LTL conjugates. This product comes in a lyophilized form and is stored at -20°C. Calcium and manganese ions are required for binding.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg /ml in 1XPBS, pH 7.4. Less than 10mg/mL may be required to agglutinate neuraminidase-treated type O human erythrocytes.
Technical Specifications
| Abbreviation | : | LTL |
| Material Source: | : | Asparagus pea |
| Conjugate: | : | None |
| Molecular Weight: | : | 107 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | O |
| Preferred Sugar Specificity: | : | α-Fucose |
| Inhibiting or Eluting Sugar: | : | L-Fucose |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Blotting, Cancer biomarker, Cell typing, Bioconjugation, diagnostic tool (Distinguish pathogenic trypanosomes), Glycobiology.
References
- Allen HJ, Johnson EA. A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus. Carbohydr Res. 1977 Oct;58(2):253-65. doi: 10.1016/s0008-6215(00)84352-9. PMID: 912690.
- Yariv J, Kalb AJ, Katchalski E. Isolation of an L-fucose binding protein from Lotus tetragonolobus seed. Nature. 1967 Aug 19;215(5103):890-1.
