Phaseolus vulgaris Lectin (PHA-E) - Pure
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Product Description
Affinity-purified Phaseolus vulgaris lectin (PHA-E) is a tetrameric glycoprotein responsible for the erythroagglutinating properties of the PHA fraction. It has carbohydrate specificity for oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E binds to human erythrocytes and lymphocytes, with a preference for blood group A (-SA), and lymphocytes have five times more PHA-E receptors than erythrocytes. The crystal structure of ligand-free PHA-E features a typical legume lectin fold with two β-sheets and two α-helices, containing one GlcNAc residue from the N-linked glycan. An asparagine-linked glycopeptide inhibits PHA-E-induced agglutination and mitogenicity. PHA-E binds di-galactosylated and bisected N-glycans, making it useful for detecting bisecting GlcNAc- and Gal-bearing glycoproteins.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. 1-10 μg/ml of PHA-E can agglutinate human or rabbit erythrocytes.
Technical Specifications
| Abbreviation | : | PHA-E |
| Material Source: | : | Kidney bean |
| Conjugate: | : | None |
| Concentration: | : | 5 mg, 10 mg |
| Molecular Weight: | : | 122 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A(-SA) |
| Preferred Sugar Specificity: | : | Complex N-Glycans |
| Inhibiting or Eluting Sugar: | : | Bovine Thyroglobulin, Acetic Acid |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence study, Bioconjugations, Blotting, Cell typing, HNK1 binding study, Glycobiology.
References
- RIGAS DA, OSGOOD EE. Purification and properties of the phytohemagglutinin of Phaseolus vulgaris. J Biol Chem. 1955 Feb;212(2):607-15.
- Wang P, Leng X, Duan J, Zhu Y, Wang J, Yan Z, Min S, Wei D, Wang X. Functional Component Isolated from <i>Phaseolus vulgaris</i> Lectin Exerts In Vitro and In Vivo Anti-Tumor Activity Through Potentiation of Apoptosis and Immunomodulation. Molecules. 2021 Jan 18;26(2):498.
- Baumgartner P, Raemaekers RJ, Durieux A, Gatehouse A, Davies H, Taylor M. Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Protein Expr Purif. 2002 Dec;26(3):394-405.
- Green ED, Baenziger JU. Oligosaccharide specificities of Phaseolus vulgaris leukoagglutinating and erythroagglutinating phytohemagglutinins. Interactions with N-glycanase-released oligosaccharides. J Biol Chem. 1987 Sep 5;262(25):12018-29.
- Jiang B, Yuan Y, Zhang X, Feng Z, Liu C. Separation and Enrichment of Lectin from Zihua Snap-Bean (Phaseolus vulgaris) Seeds by PEG 600-Ammonium Sulfate Aqueous Two-Phase System. Molecules. 2017 Sep 22;22(10):1596.
- Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (<i>Phaseolus vulgaris</i>) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
Technical Documentation
Product Information SheetSafety Data Sheet (SDS)
Certificates of Analysis (COA)
