Ulex europaeus Lectin (UEA I) - Pure

Product Description

Ulex europaeus agglutinin (UEA I) is affinity-purified from gorse seeds and separated from crude extract using affinity chromatography. Composed of two subunits, this glycoprotein has no mitogenic activity and primarily agglutinates type O erythrocytes, with lesser activity toward A2 erythrocytes. UEA I exhibits strong reactivity with α(1,2)-linked fucose residues but minimal or no binding to α(1,3) or α(1,6)-linked fucose. It cannot bind internal fucose structures, and the presence of an internal α(1,3)-linked fucose on H type 2 oligosaccharides reduces lectin affinity for this blood group antigen by three-fold. UEA I has been utilized as a marker for vascular endothelium and to identify endothelial cells in benign vascular lesions more specifically than Factor VII-related antigen antibodies. Additionally, UEA I-coated magnetic beads are employed to isolate endothelial cells associated with micro-vessels for cell culture and to differentiate between angiosarcomas and epithelial tumors.

Recommended Usage: Recommended dilutions of 0.5 - 10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. Less than 4 mg/ml will agglutinate human type O erythrocytes. Less than 0.5 mg/ml will agglutinate neuraminidase treated erythrocytes