Triticum vulgaris Lectin (WGA) - Pure
Price: Regular price $178.41
Product Description
Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells.
Similar to insulin, WGA enhances the rate of glucose oxidation in isolated fat cells and is used for isolation and fractionation of insulin receptors. A recent time-of-addition study made it evident that WGA inhibits infection with SARS-CoV-2 by inhibiting replication when the lectin is incubated with the virus and added to cells during infection. The data from the same study indicated the mode of action is an interaction between WGA and the viral envelope, causing neutralization of the virus. It is possible this occurs via the highly glycosylated spike proteins. WGA has been used in oral squamous cell carcinoma staining techniques to test the degree of cell cohesion. It has been shown to inhibit melanoma cell proliferation in a dose-dependent manner. Cytotoxic activity on cancer cell lines is characteristic of WGA. The effects of its binding have been studied in a plethora of cancers including but not limited to hepatoma, choriocarcinoma, osteosarcoma, and some pancreatic cancers.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 1XPBS. 10 µg/ml will agglutinate human erythrocytes nonspecifically.
Technical Specifications
| Abbreviation | : | WGA |
| Material Source: | : | Wheat |
| Conjugate: | : | None |
| Concentration: | : | 10 mg, 25 mg, 50 mg |
| Molecular Weight: | : | 36 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A, B, O |
| Preferred Sugar Specificity: | : | GlcNAc |
| Inhibiting or Eluting Sugar: | : | (GlcNAc)5 |
| Divalent Ions: | : | None |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Blotting, ELISA, Bioconjugations, Cell typing, Antiviral, Antiproliferative, Neuronal tracing, Glycobiology.
References
- Robert Bloch and Max M. Burger. purification of wheat germ agglutinin using affinity chromatography on chitin. biochemical and biophysical research communications, March1974, Vol. 58, No. 1.
- Allen AK, Neuberger A, Sharon N. The purification, composition and specificity of wheat-germ agglutinin. Biochem J. 1973 Jan;131(1):155-62.
- Wright CS. Crystal structure of a wheat germ agglutinin/glycophorin- sialoglycopeptide receptor complex. Structural basis for cooperative Lectin-cell binding. J Biol Chem. 1992 Jul 15;267(20):14345-52.
- Schwefel D, Maierhofer C, Beck JG, Seeberger S, Diederichs K, Möller HM, Welte W, Wittmann V. Structural basis of multivalent binding to wheat germ agglutinin. J Am Chem Soc. 2010 Jun 30;132(25):8704-19.
Technical Documentation
Product Information SheetSafety Data Sheet (SDS)
Certificates of Analysis (COA)
