Artocarpus integrifolia Lectin (AIA) - Semi-Pure
Price: Regular price $70.38
Product Description
Artocarpus integrifolia Lectin (AIA) is purified by fractionation and has a tetrameric two-chain structure consisting of four identical protomers, with an isoelectric point of pH 7.8. This lectin exhibits specificity for blood group types O (+SA) and T. Jacalin is classified as a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification provides Jacalin with a novel carbohydrate-binding site at the N terminus of the α-chain. The carbohydrate-binding specificity of AIA for the Thomsen-Friedenreich antigen (TF-Galß1-3GalNAc) suggests antitumor potential, as this antigen is present in over 85% of human carcinomas. Jacalin has demonstrated antiproliferative effects on colonic epithelial cells when administered to carcinogen-exposed mice, indicating potential in colorectal cancer treatment. Possible anticancer pathways include COX-2 inhibition and apoptotic or necrotic cell death induced by high levels of TNF-α from Jacalin-activated macrophages. Additionally, Jacalin may be explored in SARS-CoV2 research, as it can recognize the receptor-binding domain (RBD) of the virus and induce a conformational change. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal, and 2-deoxy-α-D-galactose. This product is available in a stable lyophilized form.
Recommended Usage: Recommended dilutions of 10-60 µg / ml in 1XPBS. Less than 1 mg / ml can agglutinate neuraminidase treated by human erythrocytes.
Technical Specifications
| Abbreviation | : | AIA |
| Material Source: | : | Jackfruit Seed |
| Conjugate: | : | None |
| Concentration: | : | 5 mg/ml |
| Molecular Weight: | : | 53 kDa |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galß1-3GalNAc |
| Inhibiting or Eluting Sugar: | : | Galactose or Melibiose |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Mitogenic stimulation. Immunocytochemistry. Cell typing. Glycobiology.
References
- Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (<i>Phaseolus vulgaris</i>) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
- S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
- Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
- Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
- T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.