Griffonia simplicifolia Lectin (GSL II) - Pure
Price: Regular price $113.85
Product Description
GSL II is an affinity-purified tetramer composed of a single 30 kDa subunit. Notably, it possesses insecticidal structure/function and is the first GlcNAc-binding legume lectin demonstrated to have insecticidal activity. This lectin exhibits carbohydrate specificity for α-Gal and α-GalNAc, specifically recognizing terminal GlcNAc. It elutes with galactose or N-acetylgalactosamine, and increasing the number of N-acetylglucosamine residues beyond two does not enhance affinity. GSL II uniquely recognizes exclusively α- or β-linked N-acetylglucosamine residues at the nonreducing terminal oligosaccharides and shows a preference for blood group A (-SA) over blood group B (-SA).
Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS pH 7.4. 5-10 mg/ml will agglutinate Tk polyagglutinable cells.
Technical Specifications
| Abbreviation | : | GSL II |
| Material Source: | : | Bandeiraea |
| Conjugate: | : | None |
| Molecular Weight: | : | 120 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | ≥ 95% |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A (-SA)>>B (-SA) |
| Preferred Sugar Specificity: | : | α -N-Acetylglucosamine, β-N-Acetylglucosamine |
| Inhibiting or Eluting Sugar: | : | Chitin Hydrolysate or N-Acetylglucosamine |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Bioconjugation, Golgi staining, Uterine blood vesels & carcinoma marker, Blotting, Cell typing. Glycobiology.
References
- Lamb JE, Shibata S, Goldstein IJ. Purification and Characterization of Griffonia simplicifolia Leaf Lectins. Plant Physiol. 1983 Apr;71(4):879-87.
- Hayes CE, Goldstein IJ. An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization. J Biol Chem. 1974 Mar 25;249(6):1904-14.
- Delmotte FM, Goldstein IJ. Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography. Eur J Biochem. 1980 Nov;112(2):219-23.
