Griffonia simplicifolia Lectin (GSL I) - Pure
Price: Regular price $111.78
Product Description
Griffonia simplicifolia lectin (GSL I) is affinity-purified from a woody climbing shrub native to Africa and consists of two subunits, A and B, forming tetrameric structures with five isolectins. GSL I binds to terminal β-D-galactosyl and terminal N-acetyl-β-D-galactosaminyl residues. The A-rich variant preferentially agglutinates blood group A erythrocytes, indicating specificity for N-acetylgalactosamine residues, while the B-rich variant preferentially agglutinates blood group B cells, demonstrating specificity for β-galactose residues. GSL I exhibits a stronger affinity for blood group B compared to A1. Additionally, it binds various glycoproteins, including laminin, and highlights specific glycosylation patterns in endothelial cells of mouse tissues.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS pH 7.4. Rabbit or human erythrocytes will agglutinate by 1 – 20 μg / ml of GSLI.
Technical Specifications
| Abbreviation | : | GSL I |
| Material Source: | : | Bandeiraea |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 5 mg |
| Molecular Weight: | : | 114 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off white to brown |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | B>A1 |
| Preferred Sugar Specificity: | : | GalNAc, Galactose |
| Inhibiting or Eluting Sugar: | : | Methyl α-D-Galactopyranoside, Melibiose |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Blotting, Mice endothelial cell marker, Cancer biomarker, Cell typing, Bioconjugation, Glycobiology.
References
- Lamb JE, Shibata S, Goldstein IJ. Purification and Characterization of Griffonia simplicifolia Leaf Lectins. Plant Physiol. 1983 Apr;71(4):879-87.
- Hayes CE, Goldstein IJ. An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization. J Biol Chem. 1974 Mar 25;249(6):1904-14.
- Delmotte FM, Goldstein IJ. Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography. Eur J Biochem. 1980 Nov;112(2):219-23.
