Concanavalin A Lectin (Con A) - Alexa Fluor 594

Product Description

Concanavalin A Lectin (Con A) is isolated from jack beans (Canavalia ensiformis) and purified by affinity chromatography. Con A is composed of identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues) and while above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. It has a carbohydrate specificity towards α-mannose and α-glucose and elutes with MeαMan + MeαGlc. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells which aggregate by Con A (normal white cells do not). Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane.

Concanavalin A Lectin (Con A) conjugated with Alexa Fluor™ 594 is a bright, red fluorescent dye designed for stable signal generation in imaging and flow cytometry. This conjugate is water soluble and pH-insensitive from pH 4 to pH 10, providing versatility for various assays. The Alexa Fluor™ 594 conjugate enhances detection performance for cellular labeling, making it suitable for a wide range of research applications.

Recommended Usage: Recommended dilutions of 0.5- 10 µg / ml in 1XPBS. More than 10 µg/mL may be required to give visible agglutination of neuraminidase treated human erythrocytes.