Concanavalin A Lectin (Con A) - Texas Red

Product Description

Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). While above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds two metal ions per monomer: transition metals, Mn2+ and Ca2+. Both ions are required for binding with optimum activity at a pH 7.0. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. Texas Red is a red-fluorescent dye that, when bound to Concanavalin A (Jackbean) Lectin (Con A), reveals the binding pattern of this lectin in cellular imaging applications. The emission spectra of Texas Red and fluorescein isothiocyanate (FITC) have minimal overlaps, making this combination ideal for dual-labeling experiments. Compared to other dyes like fluorescein, rhodamine dyes such as Texas Red are more photostable and less sensitive to pH changes.

Recommended usage: Recommended dilutions of 0.5 – 10 µg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. More than 10 µg/ml can agglutinate neuraminidase treated human erythrocytes.