Arachis hypogaea Lectin (PNA) - Biotinylated

Product Description

A

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA.

Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Arachis hypogaea (Peanut) Lectin (PNA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Recommended Usage: Recommended dilutions are 0.5 – 10 µg / ml in 20mM Tris-HCl pH 7.5 containing 1mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. Less than 1 µg / ml will agglutinate neuraminidase treated erythrocytes (human, porcine).