Arachis hypogaea Lectin (PNA) - DyLight 488

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3) GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This Lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

DyLight 488 is a green-fluorescent dye used to visualize the binding pattern of Arachis hypogaea (Peanut) Lectin (PNA) in cellular imaging and flow cytometry. It operates effectively over a broad pH range (pH 4-9) and offers superior brightness and photostability compared to other green-fluorescent dyes, including FITC, making it an excellent choice for various applications.

Recommended Usage: Recommended dilutions are 0.5 – 10 µg / ml in 20 mM Tris Buffer containing 1mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. Less than 1 µg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.