Arachis hypogaea Lectin (PNA) - Alexa Fluor 750

Product Description

21511456-1     Arachis hypogaea Lectin (PNA) - Alexa Fluor 750

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography and consists of four subunits with a size of approximately 27 kDa each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3) GalNAc and de-sialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Although extracts of Arachis hypogaea do not agglutinate untreated or trypsin-treated human erythrocytes, PNA has been known to agglutinate neuraminidase-treated human red blood cells. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This Lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Arachis hypogaea Lectin (AHL) conjugated with Alexa Fluor™ 750 is a bright and photostable near-IR dye that is spectrally similar to Cy7. Designed for stable signal generation in imaging and flow cytometry, this conjugate is water soluble and pH-insensitive from pH 4 to pH 10. The fluorescence of Alexa Fluor™ 750 is not visible to the human eye but can be readily detected by most imaging systems, making it an excellent choice for sophisticated cellular labeling and detection applications.