Amaranthus caudatus Lectin (ACL/ACA) - DyLight 488
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Product Description
Affinity-purified Amaranthus caudatus (ACL/ACA) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the “T” antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.
DyLight 488 is a green-fluorescent dye used to visualize the binding pattern of ACL in cellular imaging and flow cytometry. It performs effectively across a broad pH range (pH 4-9) and offers superior brightness and photostability compared to other green fluorophores, including FITC.
Recommended Usage: Recommended dilutions to use 0.5 – 10 µg / ml in 1XPBS. Less than 0.5 µg will agglutinate neuraminidase treated erythrocytes.
Technical Specifications
| Abbreviation | : | ACL/ACA |
| Conjugate: | : | DyLight® 488 |
| Concentration: | : | 1 mg/ ml |
| Molecular Weight: | : | 54 kDa |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Disialylated Fetuin |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Glycobiology, Biomarker.
References
1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.