Allium sativum Lectin (ASA) - Texas Red
Price: Regular price $222.53
Product Description
Allium sativum lectin (ASA) is isolated from garlic bulbs and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. The binding site can withstand terminal sialic acid and galactose residues. The blood group specificity of ASA is towards rabbit erythrocytes and weak interaction with human erythrocytes. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.
Texas Red is a red-fluorescent dye that, when bound to ASA, effectively reveals the binding pattern in cellular imaging applications. With minimal overlap between its emission spectra and FITC, this combination is ideal for dual-labeling experiments. Additionally, Texas Red offers greater photostability and is less sensitive to pH changes compared to other dyes like fluorescein.
Application: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Glycobiology.
Recommended Usage: Recommended dilution is 0.5 – 10 µg/ml. 1XPBS can be used to dilute the stock. Less than 0.5 ug /ml will agglutinate rabbit erythrocytes.
Technical Specifications
| Abbreviation | : | ASA |
| Material Source: | : | Garlic |
| Conjugate: | : | Texas Red |
| Concentration: | : | 2 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Mannose |
| Inhibiting or Eluting Sugar: | : | α(1,3)-Linked Mannosyl Units |
| Fluorescence: | : | Red |
| Divalent Ions: | : | None Required |
| Excitation: | : | 595 nm |
| Emission: | : | 615 nm |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
