Allium sativum Lectin (ASA) - Cy5
Price: Regular price $169.74
Product Description
Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.
Cy5, when bound to Allium sativum Lectin (ASA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlapping with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.
Application: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Anti-cancer, Glycobiology.
Recommended usage: 1XPBS can be used for reconstitution/dilution. Recommended dilution range is 0.5-10 µg / ml.
Technical Specifications
| Abbreviation | : | ASA |
| Material Source: | : | Garlic |
| Conjugate: | : | Cyanine 5 (Cy5) |
| Concentration: | : | 1 mg/mL |
| Molecular Weight: | : | N/A |
| Molecular Formula: | : | N/A |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Mannose |
| Inhibiting or Eluting Sugar: | : | α(1,3)-Linked Mannosyl Units |
| Fluorescence: | : | Red |
| Divalent Ions: | : | None Required |
| Excitation: | : | 650 nm |
| Emission: | : | 670 nm |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
