Phaseolus vulgaris Lectin (PHA-E) - Pure
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Product Description
Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. The crystal structure of a ligand-free PHA-E has a typical legume lectin fold characterized by two anti-parallel ?-sheets and two short ?-helices, and contains one GlcNAc residue of the N-linked glycan. Asparagine linked erythrocytes glycopeptide is an inhibitor of PHA-E induced agglutination and mitogenicity, and becomes inactive if treated with ?-galactosidase. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins.
Technical Specifications
| Abbreviation | : | PHA-E |
| Material Source: | : | Kidney bean |
| Conjugate: | : | None |
| Molecular Weight: | : | 126 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A(-SA) |
| Preferred Sugar Specificity: | : | Complex N-Glycans |
| Inhibiting or Eluting Sugar: | : | Bovine Thyroglobulin, Acetic Acid |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Erythroagglutinin studies, Binding of HNK-1, Model for carbohydrate recognition of proteins
References
Technical Documentation
Product Information SheetSafety Data Sheet (SDS)
Certificates of Analysis (COA)
