Vigna radiata Lectin (VRA) - AP (Alkaline Phosphatase)

Vigna radiata Lectin (VRA) - AP (Alkaline Phosphatase)

Price: Regular price $234.63

SKU#: 21510941-1

Product Description

Affinity purified VRA agglutinates rabbit blood cells and is galactose-specific. Amino acid analysis of the purified mung bean lectin by reverse phase HPLC revealed that it contains glutamate in highest proportion followed by aspartate and histidine, indicating that it has a good zinc binding potential. Binding VRA with zinc improves overall stability and efficacy of the lectin.Alkaline phosphatase (AP) is conjugated to Vigna radiata Lectin (VRA) to show the binding of VRA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: VRA
Material Source:: Mung bean
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: 85-92%
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Galactose
Inhibiting or Eluting Sugar:: α-Galactose
Divalent Ions:: None Required
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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