Vicia ervilia Lectin (VEA) - Pure
Price: Regular price $307.66
Product Description
Vicia ervilia agglutinin (VEA) is isolated from bitter vetch seeds and purified by affinity chromatography. It has a molecular weight of 60,000 and is composed of four subunits. It has an isoelectric point between pH 5.8 and pH 7.8. Sugar analysis of VEA has shown negligible amounts of carbohydrate (about 1 mol glucose per unit of MW). VEA agglutinates human erythrocytes of A, B, and O type, and activity is intact at pH 3 _Ñà10. This lectin has possible utility as a biospecific adsorbent especially for virus purification, and material recovered from the biospecific adsorption step is homogenous at neutral pH.
The agglutination is inhibited by the presence of glucose, fructose, D-mannose, ?-methylmannoside, N-acetyl glucosamine, sucrose, maltose, melezitose and trehalose; mannose and trehalose are the strongest inhibitors. No inhibition is caused by galactose, galactoseamine, N-acetyl galactoseamine, fucose, N-acetyl mannoseamine, cellobiose, lactose or sialic acid.
This product comes in a lyophilized form and is stable for more than three years when stored below -20ëC. Reconstitute with 2 ml deionized water.
Technical Specifications
Abbreviation | : | VEA |
Material Source: | : | Bitter vetch |
Conjugate: | : | None |
Molecular Weight: | : | 60 kDa |
Shelf Life: | : | 2 years |
Blood Group Specificity: | : | Rabbit |
Preferred Sugar Specificity: | : | Mannose, Glucose |
Inhibiting or Eluting Sugar: | : | Mannose, Glucose |
Lyophilized or Liquid | : | Lyophilized |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |