Vicia ervilia Lectin (VEA) - Pure

Vicia ervilia Lectin (VEA) - Pure

Price: Regular price $307.66

SKU#: 21510268-1

Product Description

Vicia ervilia agglutinin (VEA) is isolated from bitter vetch seeds and purified by affinity chromatography. It has a molecular weight of 60,000 and is composed of four subunits. It has an isoelectric point between pH 5.8 and pH 7.8. Sugar analysis of VEA has shown negligible amounts of carbohydrate (about 1 mol glucose per unit of MW). VEA agglutinates human erythrocytes of A, B, and O type, and activity is intact at pH 3 _ÑÐ 10. This lectin has possible utility as a biospecific adsorbent especially for virus purification, and material recovered from the biospecific adsorption step is homogenous at neutral pH.

The agglutination is inhibited by the presence of glucose, fructose, D-mannose, ?-methylmannoside, N-acetyl glucosamine, sucrose, maltose, melezitose and trehalose; mannose and trehalose are the strongest inhibitors. No inhibition is caused by galactose, galactoseamine, N-acetyl galactoseamine, fucose, N-acetyl mannoseamine, cellobiose, lactose or sialic acid.

This product comes in a lyophilized form and is stable for more than three years when stored below -20ëC. Reconstitute with 2 ml deionized water.

Technical Specifications

Abbreviation: VEA
Material Source:: Bitter vetch
Conjugate:: None
Molecular Weight:: 60 kDa
Shelf Life:: 2 years
Blood Group Specificity:: Rabbit
Preferred Sugar Specificity:: Mannose, Glucose
Inhibiting or Eluting Sugar:: Mannose, Glucose
Lyophilized or Liquid: Lyophilized
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

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