Ulex europaeus agglutinin (UEA I) is affinity purified isolated from gorse seeds and separated from crude extract by affinity chromatography. It is consists of two subunits. The lectin is a glycoprotein that has no mitogenic activity to agglutinates type O erythrocytes, and of a lesser degree to individuals with A2 erythrocytes.UEA I reacts strongly with Î±(1,2) linked fucose residues but poorly or not at all with Î±(1,3) or Î±(1,6)-linked fucose. UEA I is unable to bind internal fucose structures and the presence of an internal Î±(1,3)-linked fucose on the H type 2 oligosaccharide decreases lectin affinity for this blood group antigen by 3-fold.UEA I has been used as a marker for vascular endothelium and used to identify endothelial cells of benign vascular lesions more specifically than antibodies Factor VII-related antigen. UEA I coated magnetic beads have been used to isolate endothelial cells associated with micro-vessels for subsequent cell culture. This lectin has also been used to differentiate between angiosarcomas and epithelial tumor.MicroPassâ„¢ microcolumns are prepared by conjugating SeparoporeÂ® 4B with affinity purified (AffyPureâ„¢) UEA I. These microcolumns are used for MS and proteomic analysis to analyze <100µL samples.