Triticum vulgaris Lectin (WGA) - Colloidal Gold

Triticum vulgaris Lectin (WGA) - Colloidal Gold

Price: Regular price $115.81

SKU#: 21510844-1

Product Description

     Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. 

   Similar to insulin, WGA enhances the rate of glucose oxidation in isolated fat cells and is used for isolation and fractionation of insulin receptors. A recent time-of-addition study made it evident that WGA inhibits infection with SARS-CoV-2 by inhibiting replication when the lectin is incubated with the virus and added to cells during infection. The data from the same study indicated the mode of action is an interaction between WGA and the viral envelope, causing neutralization of the virus. It is possible this occurs via the highly glycosylated spike proteins. WGA has been used in oral squamous cell carcinoma staining techniques to test the degree of cell cohesion. It has been shown to inhibit melanoma cell proliferation in a dose-dependent manner. Cytotoxic activity on cancer cell lines is characteristic of WGA. The effects of its binding have been studied in a plethora of cancers including but not limited to hepatoma, choriocarcinoma, osteosarcoma, and some pancreatic cancers. 

     Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of WGA. Colloidal gold labeled lectins can be viewed via electron microscopy

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical

 

Specifications:

  • SourceTriticum vulgaris (Wheat germ)
  • Activity: 10 µg/ml will agglutinate human erythrocytes nonspecifically.
  • Carbohydrate Specificity: N-Acetylglucosamine
  • Inhibitory Carbohydrate: Chitin hydrolysate or 500 mM N-Acetylglucosamine
  • Mitogenic Activity: Yes
  • Divalent Ions Required: Ca2+, Mn2+
  • Conjugation: Colloidal Gold

 

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

 

Technical Specifications

Abbreviation: WGA
Material Source:: Wheat
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
Divalent Ions:: Ca++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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