The Intense™ polymerizedAlkaline Phosphatase enzyme with polymer chemistries was used to create a highly sensitiveBiotin-free one step detection system. This Intense™ highly active enzyme is attached to the affinity purified secondary antibodies to produce a highly sensitive and low background reagent. ThisAlkaline Phosphatase Polymer limits steric interference and provides enhanced accessibility to the target antigens. The resulting crisp staining of antibody targets, especially nuclear and membrane antigens with greater sensitivity than conventionalAlkaline Phosphatase antibody conjugates can be used with a variety of AP substrate to obtain a desired color in immunohistochemistry applications. Kit components include: â€šÃ Ã¶âˆšÃ² 15mL Intense™-AP (alkaline phosphatase) Polymer Anti-Rat IgG, 15mL â€šÃ Ã¶âˆšÃ² Normal Goat Serum for blocking 15mL, 2.5% ThisKit will stain up to 100-150 sections.