The versatile Avidin: Biotin complex interaction allows this system to be modular, and along with the appropriate selection of secondary antibodies, it can accommodate a wide array of primary antibodies and tissues from different species. The Scorpion™ Avidin: BiotinKit is easy to use and economical for any immunohistochemistry (IHC) and immunocytochemistry (ICC) applications. The choice of Peroxidase substrates produces sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the Scorpion™ universal systems, make it a preferred choice in many applications, such as neural tissue where it gives more consistent labeling. The following features of thisKit make it preferred reagent in manyHRP detection applications:
‚àö√ò Fast Procedure: Staining in less than 30 minutes after primary antibody labeling.
‚àö√ò High sensitivity and low background.
‚àö√ò Biotinylated Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.
‚àö√ò ReadyGo™: The working solutions are prediluted and stabilized for staining, saving time.
ThisKit contains sufficient reagents to stain approximately 300-500 fresh or paraffin embedded sections.
Kit Components include:
Each of the stabilized, prediluted reagents are supplied in ReadyGo™ dropper bottles.
‚àö√ò Peroxidase-streptavidin complex, ReadyGo™, 50mL Note: The UniversalBiotinylated Anti-Mouse/ Rabbit IgG secondary antibody included in thisKit contains bothBiotinylated anti-mouse IgG and anti-rabbit IgG to detect both rabbit and mouse primary antibodies in a single step. This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with the IgGHRP present in tissues of these species.