The Tyramid Signal Amplification (TSA) system in combination with polyHRP produces crisp, highly sensitive, and specific staining with low background. This TSA Staining System utilizes catalytic activity ofHRP to generate high density labeling and can increase the detection ability up to 100-fold. It allows detection of sub microgram amounts of primary antibodies. ThisKit contains ready to use polyHRP-coupled anti-mouse IgG which is purified and extensively cross-adsorbed to ensure high sensitivity and low background. The TSA detection method can be applied to a variety of histochemical preparations including cryostat frozen sections, paraffin-embedded sections and plastic embedded sections. In this application, due to the high sensitivity, it is essential to remove endogenous peroxidase activity before applying these reagents. ThisKit can be used withBiotinyl Tyramine (Tyramide) or any other fluorophore Tyramides. Also, to reduce further background a TCP Blocking reagent is recommended (bioWORLD SKU ).