The sensitivity of Western blot detection can be significantly increased by using signal amplification detection procedure. OurBiotin: Avidin andBiotin/Streptavidin detection systems introduce many enzyme molecules to the target site amplifying the signal while keeping a low background. The Scorpion™Alkaline PhosphataseHRP Amplification Kit allows detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent or a chromogenicHRP substrate. The sensitivity of this system allows for the detection of target proteins in the picogram range.
TheKit contains enough reagents to develop up to twenty 100 cm2 membrane blots.
Kit Components include:
‚àö√ò Casein Blocking Solution, 250mL, 5X
‚àö√ò PolyHRP-conjugated Goat Anti-Rabbit IgG
‚àö√ò 0.5mL Reagent A (Avidin D) and 0.5mL Reagent B (BiotinylatedHRP)
‚àö√ò Intensive Chemiluminescent (ICL) Substrate A, 100mL