Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.The blood group A-active trisaccharide containing L-fucosyl group linked Î±1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc Î±1-3Gal), which suggests steric hindrance to binding by the Î±-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.MicroPass™ microcolumns are prepared by conjugating Separopore® 4B with affinity purified (AffyPure™) Sambucus nigra Lectin (SNA/EBL II). These microcolumns are used for MS and proteomic analysis to analyze of <100µL samples.