ReadyGo™ Red AP Substrate Kit is a formulation that produces a bright red reaction product utilizing AP conjugates in IHC applications. This ReadyGo™ Substrate Kit consists of 100mL of diluent and concentrated stock solutions of Red-AP Substrate in a convenient dropper bottle format. This AP substrate produces a heat stable reaction product that is also highly fluorescent and non-fading. Sections stained with Red AP chromogen can be dehydrated, cleared and permanently mounted in organic media. This formulation can be used unaccompanied or in combination with otherAlkaline Phosphatase or peroxidase substrates for multiple labeling applications. AP-Red substrate can be used for both formulations and for either manual or automated staining procedures. Additionally, this reagent can be used on blots.
This red fluorescence of the AP-red reaction products is visible using DyLight‚àö√ú 594,Texas Red‚àö√ú or TRITC filter systems. The fluorescence is bright in dehydrated permanently mounted sections and is stable without photobleaching. Excitation: 350 ‚àö¬± 560nm with emission at 560nm providing a dynamic range.