Enables Western Blotting preparation and development in less than 90 minutes. The Rapid Western Blotting Kit provides reagents for all blotting steps from gel transfer, blocking and a combined 1º and 2º antibody incubation step. Users provide the primary antibody and chemiluminescent substrates.
One convenient kit includes all of the following specialized reagents:
Rapid Transfer Buffer, 10X
Transfer from gel to PVDF or nitrocellulose membrane in 10-20 minutes, Use with wet or semi-dry transfer apparatus, Methanol-free, non-hazardous formulation
RapidBlock™ Solution, 10X
Blocks PVDF and nitrocellulose membranes in just 5 minutes, Protein-free formulation reduces cross-reactivity, Enhances chemiluminescent signal
Rapid Antibody Diluent, 10X
One-step combined primary and secondary antibody incubation reduces total incubation time to 30-45 minutes, Secondary HRP Conjugated Antibodies, Choose from either anti-mouse or anti-rabbit antibodies
Rapid Wash Solution, 20X
Reduces wash time to a total of 15 minutes
Kits are supplied for either standard wet or semi-dry transfer apparatus and with either anti-mouse or anti-rabbit secondary antibodies.