Synonym(s): Western blotting kit
The Rapid Western Blotting Kit provides reagents for all blotting steps from gel transfer, blocking and a combined 1° and 2° antibody incubation step. Users provide the primary antibody and chemiluminescent substrates.
Enables Western Blotting preparation and development in less than 90 minutes.
One convenient kit includes all of the following specialized reagents:
Rapid Transfer Buffer, 10X
Transfer from gel to PVDF or nitrocellulose membrane in 10-20 minutes
Use with wet or semi-dry transfer apparatus
Methanol-free, non-hazardous formulation
RapidBlock™ Solution, 10X
Blocks PVDF and nitrocellulose membranes in just 5 minutes
Protein-free formulation reduces cross-reactivity
Enhances chemiluminescent signal
Rapid Antibody Diluent, 10X
One-step combined primary and secondary antibody incubation reduces total incubation time to 30-45 minutes
Secondary HRP Conjugated Antibodies
Choose from either anti-mouse or anti-rabbit antibodies
Rapid Wash Solution, 20X
Reduces wash time to a total of 15 minutes
Kits are supplied for either standard wet or semi-dry transfer apparatus and with either anti-mouse or anti-rabbit secondary antibodies.