Psophocarpus tetragonolobus Lectin (PTL/PTA II) - TRITC (Rhodamine)

Psophocarpus tetragonolobus Lectin (PTL/PTA II) - TRITC (Rhodamine)
Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA II) - TRITC (Rhodamine)
Product image 1Psophocarpus tetragonolobus Lectin (PTL/PTA II) - TRITC (Rhodamine)
Product image 2Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA II) - TRITC (Rhodamine)

Price: Regular price $122.03

SKU#: 21511150-1

Product Description

Psophocarpus tetragonolobus lectin (PTL II, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by fractionation. The seed contains two lectins in which PTL II is the acidic of the two. This lectin agglutinates trypsinized and desialized erythrocytes of all three blood group types (A, B, and O). Nonpolar interactions play an important role in stabilization of the lectin-sugar complex. Projection of α1,3 linked Gal/GalNAc leads to steric hindrance and prevents the binding of blood group A and B reactive sugars.The unique specificity of PTL II makes it to be useful in isolation and characterization of terminally monofucosylated H-reactive oligosaccharides from those that are difucosylated or internally fucosylated.

Psophocarpus tetragonolobus Lectin (PTL/PTA II) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled PTL/PTA II can be used for cellular imaging applications.

Technical Specifications

Abbreviation: PTL/PTA II
Material Source:: Winged bean
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine, Galactose
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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