Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognize blood group A-substance derived oligosaccharides such as trisaccharide GalNAcÎ±1-3GalÎ²1-4Glc. Psophocarpus tetragonolobus Lectin (PTL/PTA I) OnePASS™ Spin Columns are standard PTL/PTA I matrix prepacked in a column which can be used via gravity flow or as a spin column to rapidly separate bound and passthrough materials.