Product DescriptionPsophocarpus tetragonolobus(psophocarpinB1, a storage protein with chymotrypsin inhibitory activity), PTL I lectin is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is thebasicof the two, with an isoelectric point greater than pH 9.5 and a molecular weight of 58,000. PTL I is a dimeric glycoprotein with specificity to GalNAc, eluting the sugar GalNAc.This lectin agglutinates trypsinized rabbit and human type A and B erythrocytes but not trypsinized human type O erythrocytes. PTL I recognizes blood group A-substance derived oligosaccharides such as trisaccharide GalNAcÃƒÂ¯Ã‚Â±1-3GalÃƒÂ¯_1-4Glc. Studies on inhibition assays of lectin-blood group A precipitation demonstrated that substance-derived oligosaccharides were the best inhibitors and had the common structure of D-GalNAc alpha(1,3)D-Gal-(beta 1,3/4) to a D-Glc, and about 8 and 4 times more active than D-GalNAc and D-GalNAc alpha(1,3)D-Gal, respectively.Reports on the thermodynamics and kinetics of the lectin suggest that it has an extended binding site, and its interactions with saccharides involvereorientationof water molecules.This product comes in a lyophilized powder form and is stored at -20ÃƒÂ«C.
Applications:GlycobiologyStaining of mouse M-cellsBlood vessel staining of A and B group individuals
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Matsuda, T., et al. Carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus).Mol Immunol.(1989) 26(2):189-95.
Patanjali, S., et al. Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus).Jbiochem. (1988) 252:625-631.
Sharma, V., et al. Cloning and sequencing of the winged bean (Psophocarpus tetragonolobus)basic agglutinin (WBA I):presenceof second glycosylation site and its implications in quaternary structure.FEBS J.(1996) 389:289-292.