Psophocarpus tetragonolobus Lectin (PTL/PTA I) - AP (Alkaline Phosphatase)

Psophocarpus tetragonolobus Lectin (PTL/PTA I) - AP (Alkaline Phosphatase)

Price: Regular price $165.84

SKU#: 21510959-1

Product Description

Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognize blood group A-substance derived oligosaccharides such as trisaccharide GalNAcα1-3Galβ1-4Glc.

Alkaline phosphatase (AP) is conjugated to Psophocarpus tetragonolobus Lectin (PTL/PTA I) to show the binding of PTL/PTA I in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: PTL/PTA I
Material Source:: Winged bean
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine, Galactose
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: None Required
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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