Pisum sativum Lectin (PSA/PSL) - Colloidal Gold

Pisum sativum Lectin (PSA/PSL) - Colloidal Gold
Pisum sativum (Pea) Lectin (PSA/PSL) - Colloidal Gold
Product image 1Pisum sativum Lectin (PSA/PSL) - Colloidal Gold
Product image 2Pisum sativum (Pea) Lectin (PSA/PSL) - Colloidal Gold

Price: Regular price $186.70

SKU#: 21510840-1

Product Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Pisum sativum Lectin (PSA/PSL). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: PSA/PSL
Material Source:: Pea
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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