Pisum sativum Lectin (PSA/PSL) - Colloidal Gold
Price: Regular price $186.70
Product Description
Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.
Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Pisum sativum Lectin (PSA/PSL). Colloidal gold labeled lectins can be viewed via electron microscopy.
Technical Specifications
| Abbreviation | : | PSA/PSL |
| Material Source: | : | Pea |
| Conjugate: | : | Colloidal Gold |
| Concentration: | : | 1 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | α-Mannose, α-Glucose |
| Inhibiting or Eluting Sugar: | : | α-Methylmannoside + α-Methylglucoside |
| Divalent Ions: | : | Ca++, Mn++ |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
