Phaseolus limensis agglutinin (LBA) is isolated from lima bean seeds and purified by affinity chromatography. LBA is composed of 30,000 Da subunits, of which two are joined by a disulfide bond. Each subunit contains a cysteine residue which is required for both itâ€™s carbohydrate- and metal-ion-binding activities. It has a carbohydrate specificity of terminal GalNAcÎ±(1,3)Gal that elutes with N-acetylgalactosamine. LBA agglutinates blood group A erythrocytes.Phaseolus limensis Lectin (LBA)Â is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled LBAÂ can be used for cellular imaging applications.