Phaseolus limensis agglutinin (LBA) is isolated from lima bean seeds and purified by affinity chromatography. LBA is composed of 30,000 Da subunits, of which two are joined by a disulfide bond. Each subunit contains a cysteine residue which is required for both it’s carbohydrate- and metal-ion-binding activities. It has a carbohydrate specificity of terminal GalNAcα(1,3)Gal that elutes with N-acetylgalactosamine. LBA agglutinates blood group A erythrocytes.
Phaseolus limensis Lectin (LBA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.