Phaseolus limensis Lectin (LBA) - AP (Alkaline Phosphatase)

Phaseolus limensis Lectin (LBA) - AP (Alkaline Phosphatase)

Price: Regular price $155.41

SKU#: 21510950-1

Product Description

Phaseolus limensis agglutinin (LBA) is isolated from lima bean seeds and purified by affinity chromatography. LBA is composed of 30,000 Da subunits, of which two are joined by a disulfide bond. Each subunit contains a cysteine residue which is required for both it’s carbohydrate- and metal-ion-binding activities. It has a carbohydrate specificity of terminal GalNAcα(1,3)Gal that elutes with N-acetylgalactosamine. LBA agglutinates blood group A erythrocytes.

Alkaline phosphatase (AP) is conjugated to Phaseolus limensis Lectin (LBA) to show the binding of LBA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: LBA
Material Source:: Lima bean
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: GalNAc α(1,3) [Fuc α(1,2)] Gal
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++ Mn++
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

Close (esc)

10% Discount!

Sign up for our mailing list below and you will receive 10% off your first order of $500.00 or more.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Search

Shopping Cart

Your cart is currently empty.
Shop now