Narcissus pseudonarcissus Lectin (NPL/NPA) - Separopore® 4B

Narcissus pseudonarcissus Lectin (NPL/NPA) - Separopore® 4B
Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Separoporeëå 4B
Product image 1Narcissus pseudonarcissus Lectin (NPL/NPA) - Separopore® 4B
Product image 2Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Separoporeëå 4B

Price: Regular price $134.55

SKU#: 21510969-1

Product Description

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.

Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.

Affinity purified Narcissus pseudonarcissus Lectin (NPL/NPA) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.

Technical Specifications

Abbreviation: NPL/NPA
Material Source:: Wild daffodil
Conjugate:: Separopore® 4B
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-D-Mannose
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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