Narcissus pseudonarcissus Lectin (NPL/NPA) - Colloidal Gold

Narcissus pseudonarcissus Lectin (NPL/NPA) - Colloidal Gold
Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Colloidal Gold
Product image 1Narcissus pseudonarcissus Lectin (NPL/NPA) - Colloidal Gold
Product image 2Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Colloidal Gold

Price: Regular price $176.27

SKU#: 21510836-1

Product Description

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.

Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Narcissus pseudonarcissus Lectin (NPL/NPA). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: NPL/NPA
Material Source:: Lent lily
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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